Yannick Degboe
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Project:
Therapeutic Modulation of Macrophage Phenotypes in Rheumatoid Arthritis
Monocytes and macrophages can display various states of activation or « polarity » illustrated by different phenotypic profiles which are usually reversible. Those are associated with distinct biological functions in inflammation and immunity. The classification of the various “alternative” M2 polarizations is a matter of debate and increasing interest. The reality of M1/M2 polarization in rheumatoid arthritis (RA) is not formally established.
Our project aim to assess
- If RA treatments can modulate in vitro the M1/M2 polarization, correcting M1 inflammatory phenotype and/or inducing M2 alternative/regulatory macrophages
- If the capacity of RA treatments to modulate in vitro M1/M2 polarization is a marker of the later in vivo therapeutic response.
Therapeutic Modulation of Macrophage Phenotypes in Rheumatoid Arthritis
Monocytes and macrophages can display various states of activation or « polarity » illustrated by different phenotypic profiles which are usually reversible. Those are associated with distinct biological functions in inflammation and immunity. The classification of the various “alternative” M2 polarizations is a matter of debate and increasing interest. The reality of M1/M2 polarization in rheumatoid arthritis (RA) is not formally established.
Our project aim to assess
- If RA treatments can modulate in vitro the M1/M2 polarization, correcting M1 inflammatory phenotype and/or inducing M2 alternative/regulatory macrophages
- If the capacity of RA treatments to modulate in vitro M1/M2 polarization is a marker of the later in vivo therapeutic response.
Animal and Cellular models:
- Human macrophages derived from primary monocytes
Techniques and Methods:
- CD14+-positive or negative selection
M-CSF or GM-CSF driven differentiation of human monocytes in macrophages
Characterization of the M1/M2 profile : Flow cytométrie, RT-PCR analysis
Characterization of the M1 / M2 secretion profile: Cytokine bead Array, ELISA