Mireille Sebbag
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Project: Macrophage polarization and their response to stimulation by immune complexes
Study of inflammatory potential of cytokine responses (balance between “proinflammatory” and “anti-inflammatory” cytokine production) of differentially polarized macrophages (using diverse cytokines or biological fluids)
Study of inflammatory potential of cytokine responses (balance between “proinflammatory” and “anti-inflammatory” cytokine production) of differentially polarized macrophages (using diverse cytokines or biological fluids)
Animal and Cellular models:
Human macrophages differentiated in vitro from purified peripheral blood monocytes (from blood buffy coats )
Human macrophages differentiated in vitro from purified peripheral blood monocytes (from blood buffy coats )
Techniques and Methods:
CD14+-positive selection
Purification of human immunoglobulins (IgG, IgA, IgM)
Monocyte/macrophage culture without adherence
CD14+-positive selection
Purification of human immunoglobulins (IgG, IgA, IgM)
Monocyte/macrophage culture without adherence