Christel Devaud
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Project:
Current project: Investigate the role of macrophages in colorectal cancer (under development)
Previous project: Impact of tumor-microenvironment in the response to immunotherapy
Studying the involvement of the immune response, in particular the immunosuppressive components, in various cancer types (renal, colon, prostate cancers…) revealed a strong implication of tumor-associated macrophages (TAMs) in the immune response that occurs in kidney primary tumors
Current project: Investigate the role of macrophages in colorectal cancer (under development)
Previous project: Impact of tumor-microenvironment in the response to immunotherapy
Studying the involvement of the immune response, in particular the immunosuppressive components, in various cancer types (renal, colon, prostate cancers…) revealed a strong implication of tumor-associated macrophages (TAMs) in the immune response that occurs in kidney primary tumors
Animal and Cellular models:
-Syngenic orthotopic pre-clinical mouse models (to assess tumor microenvironment)à implantation of mouse tumor cell lines: intra-renal (BALB/c Renca cell line), intra-caecal (BALB/c CT26 and Bl/6 MC38 cell line), intra-prostate (Bl/6 RM-1 cell line)
-transgenic mice: IL4 -/-, IL13 -/-, Foxp3DTR
-Syngenic orthotopic pre-clinical mouse models (to assess tumor microenvironment)à implantation of mouse tumor cell lines: intra-renal (BALB/c Renca cell line), intra-caecal (BALB/c CT26 and Bl/6 MC38 cell line), intra-prostate (Bl/6 RM-1 cell line)
-transgenic mice: IL4 -/-, IL13 -/-, Foxp3DTR
Techniques and Methods:
-Characterization of M1 / M2 profile in tumors through membrane markers: Flow cytometry, Classic RTPCR, RTPCR-Array, Micro-Array, RNA-sequencing.
-Characterization of the secretion M1 / M2 profile in tumors: Cytokine bead Array (Flow cytometry), ELISA, Protein membrane Array
-Co-culture of macrophages and lymphocytes (sorted from in vivo tumors)
-In vivo depletion of macrophages or activity suppression: using clodronate liposomes, anti-mouse-CCL2 antibodies, arginase inhibitor
-In vivo macrophages transfer: TAM sorted from tumors re-transferred in tumor bearing mice (intra-tumoral transfer)
-Characterization of M1 / M2 profile in tumors through membrane markers: Flow cytometry, Classic RTPCR, RTPCR-Array, Micro-Array, RNA-sequencing.
-Characterization of the secretion M1 / M2 profile in tumors: Cytokine bead Array (Flow cytometry), ELISA, Protein membrane Array
-Co-culture of macrophages and lymphocytes (sorted from in vivo tumors)
-In vivo depletion of macrophages or activity suppression: using clodronate liposomes, anti-mouse-CCL2 antibodies, arginase inhibitor
-In vivo macrophages transfer: TAM sorted from tumors re-transferred in tumor bearing mice (intra-tumoral transfer)