Céline Cougoule
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Project: Processes of human monocyte and macrophage polarization and migration during inflammation and infectious diseases
My researches are devoted to the investigation of the molecular and cellular processes controlling macrophage and epithelial cell activation during infection such as Tuberculosis.
My researches are devoted to the investigation of the molecular and cellular processes controlling macrophage and epithelial cell activation during infection such as Tuberculosis.
Animal and Cellular models:
Model of human lung organoids
Primary human monocytes
Monocyte-derived macrophages (and DC)
Murine bone marrow-derived macrophages
Peritoneal resident and thioglycollate-elicited macrophages
Murine model of acute peritonitis induced by thiolgycollate or zymosan
Model of human lung organoids
Primary human monocytes
Monocyte-derived macrophages (and DC)
Murine bone marrow-derived macrophages
Peritoneal resident and thioglycollate-elicited macrophages
Murine model of acute peritonitis induced by thiolgycollate or zymosan
Techniques and Methods:
Separation of CD14+ human monocytes
M-CSF driven differentiation of human CD14+ monocytes in macrophages
Flow cytometry analysis of macrophage polarization profiles
In vitro Assays of migration in 3D using matrices with different architectures
Gelatin-FITC degradation assay
Immunofluorescence of actin structures such as phagosomes and podosomes.
Separation of CD14+ human monocytes
M-CSF driven differentiation of human CD14+ monocytes in macrophages
Flow cytometry analysis of macrophage polarization profiles
In vitro Assays of migration in 3D using matrices with different architectures
Gelatin-FITC degradation assay
Immunofluorescence of actin structures such as phagosomes and podosomes.